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delivery in the remarks column of the specimen register and on the report form,

particularly for negative/contaminated results. The packaging material should be

autoclaved before discarding.

4. REGISTRATION OF SAMPLES

4.1 Receipt of incoming specimens

For safety and work-flow reasons, specimens should be received in the office

area of the laboratory and delivery boxes should be opened using all the

applicable biosafety procedures inside the lab.

To minimize risk of infection, the following procedures should be applied:

1. The specimen package received should be opened only in a biosafety

cabinet which may be located in a small area within the reception or in the

culture room, as they could potentially be MDR or XDR Tuberculosis. (DO

NOT OPEN ON AN OPEN BENCH AT THE LAB RECEPTION)

2. Before opening the packet, inspect the delivery box for signs of leakage; if

there is gross leakage evident, discard the box by autoclaving or burning;

do not try to open and retrieve any specimen.

3. If on gross inspection there is no leakage, disinfect the outside of the

delivery box using cotton wool or paper towels saturated with a suitable

disinfectant (5% phenol)

4. Open carefully and check for cracked or broken specimen containers or

leakage within the packaged container. If there is minimal leakage without

any gross loss of specimen, they may be processed with an asterix that

leakage was noted on receipt. (This will assist in identifying reasons for

contamination used in lab performance indicators). In case of gross

leakage, with only very little sample being available, accept the sample

and process after carefully making a note of the same as

extrapulmonary specimens are precious and repeat collection may not be

possible.

5. Check labelling of specimens with individual identification numbers and

correspondence with numbers on the accompanying list or Clinical

information forms (CIF) that are accompanying the specimens.

6. Disinfect the inside of the delivery box, wash hands after handling

specimen containers

7. Autoclave the packaging material before discarding.

8. Assign unique lab serial number to each patient.

9. Evaluate the quality of specimens and make a note as to volume (in case

of fluids), leakage, blood mixed etc. Always register the incoming

specimen in the laboratory register; each specimen receives a serial

number that should be used to label every test for the specimen. Other

data that should be reported on the laboratory register are: the date of the

receipt of the specimen, patients name, age, sex and address, the name

of the referring health centre, the reason for DST. The signature (with the

name in capitals) of the person requesting the examination should always

be present.

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